Radioimmunoassay of human calcitonin using labeled [Asu1,7]-human calcitonin analogue.
نویسندگان
چکیده
منابع مشابه
Radioimmunoassay of calcitonin in human plasma.
A sensitive, specific radioimmunoassay of calcitonin is described, suitable for measuring the hormone in peripheral plasma of normal man. With this assay we have demonstrated that calcitonin is detectable under basal Conditions and the concentrations respond to induced, and spontaneous hypercalcemia and induced hypocalcemia. The mean value for basal calcitonin of normal men was 182 pg/mI (range...
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Receptors for the hypocalcaemic hormone, calcitonin (CT), have been identified in a membrane fraction prepared from term human placentae. Binding of 125I-labelled salmon CT (125I-sCT) to the membranes was time- and temperature-dependent, saturable (Bmax. 58 +/- 11 fmol/mg of protein), of high affinity (Kd 80 +/- 21 pM) and poorly reversible. Species-specific CTs and CT analogues competed for 12...
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To increase the production level of heterologous proteins in plants, strategies such as choice of strongerpromoters, optimization of codon usage and specific localization of foreign proteins are of major concern.Calcitonin (CT), a 32 amino acid polypeptide is a powerful and specific inhibitor of bone resorption and isused to treat several human diseases. Calcitonin activity is...
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Established human lymphoid cell lines bound calcitonin with high affinity. Calcitonin binding was detectable on both B cell and T cell lines derived from patients with a spectrum of lymphoproliferative disorders. These cell lines had marked differences in binding capacity per cell even when adjusted for differences in surface area. The binding was linearly related to cell density and showed a c...
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cDNA analyses predict that the human calcitonin gene encodes the two precursor proteins of calcitonin and calcitonin gene-related peptide (CGRP). The mRNAs for the putative precursors of these peptides are derived from alternative processing of the primary transcript of this gene. The 75 amino-terminal residues of each preprohormone are predicted to be identical. We have developed the TT human ...
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ژورنال
عنوان ژورنال: Endocrinologia Japonica
سال: 1978
ISSN: 0013-7219,2185-6370
DOI: 10.1507/endocrj1954.25.617